WebApr 27, 2010 · Undifferentiated, monocytic THP-1 cells are sensitive to killing by purified Stx1, while differentiated, macrophage-like THP-1 cells are relatively refractory to the cytotoxic action of the toxin, with only approximately 30% of cells undergoing apoptosis. WebPatients with sickle cell disease (SCD) exhibit a chronic inflammatory state manifested by leukocytosis and increased circulating levels of proinflammatory cytochemokines. Our …
PARP9 and PARP14 cross-regulate macrophage activation via …
WebTHP-1 cells (human monocytic cell line) were cultured in 6-well cell culture plates (1×10 6 cells per well) and maintained in complete RPMI 1640 medium at 37°C. For differentiation … WebMar 3, 2024 · Briefly, for migration assay, 200 μL of cell suspensions without FBS (for THP-1 cells, 1 × 10 5 cells pretreated with 100 ng/mL PMA (Sigma-Aldrich, P8139) for 24 hours and rested in RPMI 1640 with 10% FBS for another 24 hours; for KFs and NFs, 3 × 10 4 cells pretreated with 500 nM AS1517499 (or DMSO control) were added to the upper 24-well ... chris thomas headteacher
THP-1 cells as a model for human monocytes - Bosshart - Annals …
WebAug 8, 2015 · Effects of IFN-γ and/or LPS on THP-1 macrophage viability after 16 or 24 h of incubation. THP-1 macrophages were incubated in RPMI medium with IFN-γ (20, 50 or … WebJan 7, 2024 · We next assessed the functionality of NK-4-treated THP-1 macrophages by analyzing cytokine production. NK-4 dose-dependently increased THP-1 macrophage secretion of the proinflammatory cytokine TNF-α after 3 days of culture (Fig. 1b). At 4 μM NK-4, TNF-α secretion increased by 36-fold compared to control cells (Fig. 1b). NK-4 did … WebNov 18, 2016 · Using the in vitro (THP-1 cells) and ex vivo (primary monocytes) approach side-by-side allowed us to make informed judgments on the usefulness of THP-1 cells as … chris thomas graphic designer los angeles