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Thp-1 macrophage-like cells

WebApr 27, 2010 · Undifferentiated, monocytic THP-1 cells are sensitive to killing by purified Stx1, while differentiated, macrophage-like THP-1 cells are relatively refractory to the cytotoxic action of the toxin, with only approximately 30% of cells undergoing apoptosis. WebPatients with sickle cell disease (SCD) exhibit a chronic inflammatory state manifested by leukocytosis and increased circulating levels of proinflammatory cytochemokines. Our …

PARP9 and PARP14 cross-regulate macrophage activation via …

WebTHP-1 cells (human monocytic cell line) were cultured in 6-well cell culture plates (1×10 6 cells per well) and maintained in complete RPMI 1640 medium at 37°C. For differentiation … WebMar 3, 2024 · Briefly, for migration assay, 200 μL of cell suspensions without FBS (for THP-1 cells, 1 × 10 5 cells pretreated with 100 ng/mL PMA (Sigma-Aldrich, P8139) for 24 hours and rested in RPMI 1640 with 10% FBS for another 24 hours; for KFs and NFs, 3 × 10 4 cells pretreated with 500 nM AS1517499 (or DMSO control) were added to the upper 24-well ... chris thomas headteacher https://wooferseu.com

THP-1 cells as a model for human monocytes - Bosshart - Annals …

WebAug 8, 2015 · Effects of IFN-γ and/or LPS on THP-1 macrophage viability after 16 or 24 h of incubation. THP-1 macrophages were incubated in RPMI medium with IFN-γ (20, 50 or … WebJan 7, 2024 · We next assessed the functionality of NK-4-treated THP-1 macrophages by analyzing cytokine production. NK-4 dose-dependently increased THP-1 macrophage secretion of the proinflammatory cytokine TNF-α after 3 days of culture (Fig. 1b). At 4 μM NK-4, TNF-α secretion increased by 36-fold compared to control cells (Fig. 1b). NK-4 did … WebNov 18, 2016 · Using the in vitro (THP-1 cells) and ex vivo (primary monocytes) approach side-by-side allowed us to make informed judgments on the usefulness of THP-1 cells as … chris thomas graphic designer los angeles

LPS induces inflammatory chemokines via TLR-4 signalling and ... - PLOS

Category:Cytokine Expression by Human Macrophage-Like Cells Derived …

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Thp-1 macrophage-like cells

(PDF) Early suppression of antiviral host response and …

WebLoss of ME-1 in THP-1 cells was also associated with reduced efferocytosis. ME-1 is a target gene of NRF2 (nuclear factor erythroid 2–related factor 2), and the authors have … WebMar 24, 2024 · To differentiate U937 and THP-1 cells into active macrophage-like cells, the cells were resuspended in culture medium containing 0.1 μM 1α, 25-dihydroxyvitamin D 3 …

Thp-1 macrophage-like cells

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WebWe also found the same trends for CARINH and IL18BP levels upon the knockdown and overexpression of IRF1 in THP-1 cells (Fig. 5j, k), again supporting the existence of a … WebThe invention discloses a construction method of a mononuclear macrophage model based on HIV-1 (human immunodeficiency virus type 1) mass infection duplication, which …

WebTHP-1 cells were treated with PMA, IFN-γ + LPS and IL-4 + IL-13, respectively, and affixed with 4% polyoxymethylene (Sigma-Aldrich) at 4°C for 15 min. ... Zhang F, Wang H, Wang X, … WebInvivoGen offers several engineered THP-1 cell lines: THP1 NF-κB cells were specifically designed for monitoring the NF-κB signal transduction pathway in a physiologically relevant cell line. They feature either the SEAP or the Lucia luciferase reporter gene. THP1-Dual™ cells allow the simultaneous study of the NF-κB pathway, by monitoring ...

WebApr 4, 2024 · Following differentiation of the THP-1 cells, MERTK upregulation was confirmed by western blot and flow cytometry. In a similar fashion, treatment with of murine bone marrow derived monocytic ... WebInvivoGen offers several engineered THP-1 cell lines: THP1 NF-κB cells were specifically designed for monitoring the NF-κB signal transduction pathway in a physiologically …

WebJul 29, 2008 · Although monocytic leukemia cell lines such as THP-1, KG-1, K562, monomac-6, and U-937 had originally been predicted to be good candidates for differentiation into DC-like cells, these leukemia cell lines have been demonstrated to be poor models for DC differentiation, most likely as they are already developed too far along …

WebMay 20, 2024 · Immunomodulatory proteins from human milk may enhance the protection and development of the infant’s gut. This study compared the immunomodulatory effects … chris thomas healerhttp://lcgdbzz.org/en/article/doi/10.3969/j.issn.1001-5256.2024.04.027 george fisher ph probeWebFigure 1 IL-17 induces M2-like phenotype in THP-1-derived macrophages. Notes: (A) THP-1-derived macrophages were treated with IL-17 at 0, 10, 50 and 100 ng/mL for 48 hours. The … george fisher pipework