Phospho fak antibody
WebThe FAK Antibody Sampler Kit provides an economical means of evaluating total FAK protein levels as well as FAK phosphorylated at specific sites. The kit contains enough primary and secondary antibody to perform two western blots with each antibody. Specificity / Sensitivity WebFocal adhesion kinase (FAK) is a non receptor protein tyrosine kinase discovered as a substrate for Src and a key element in integrin signaling. FAK plays a central role in cell spreading, differentiation, migration, cell death and acceleration of the G1 to S phase transition of the cell cycle.
Phospho fak antibody
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WebAug 2, 2024 · Rabbit antibodies against human MMP-2, MMP-9, urokinase, PI3K, and phosphorylated-PI3K were obtained from Cell Signaling Technology Inc. (Danvers, MA, USA). 4.2. Cell Culture and MTT Assay. ... (FAK), phosphorylated FAK (phospho-FAK), phosphoinositide 3-kinase (PI3K), phospho-PI3K, AKT, phospho-AKT, mTOR and phospho … WebApr 4, 2024 · Electrical stimulation for 20 h stained with anti-phospho-FAK (pY397) antibody (C) or electrical stimulation for 20 h stained with anti-phospho-c-Src (pY418) antibody (F). Bars: 20 μm. Figure 6. Changes in phosphorylated proteins, tyrosine-phosphorylated FAK (A), and tyrosine-phosphorylated c-Src (B) were examined by ELISA.
WebFAK (Focal Adhesion Kinase) is a non-receptor protein tyrosine kinase involved in signal transduction from integrin-enriched focal adhesion sites that mediate cell contact with the extracellular matrix. WebProduct name Anti-FAK (phospho Y861) antibody [EP841Y] See all FAK primary antibodies Description Rabbit monoclonal [EP841Y] to FAK (phospho Y861) Host species Rabbit Tested applications Suitable for: WB more details Unsuitable for: Flow Cyt,ICC/IF,IHC-P or IP Species reactivity Reacts with: Human Predicted to work with: Mouse, Rat Immunogen
WebInvitrogen Anti-Phospho-FAK (Tyr397) Polyclonal, Catalog # 44-624G. Tested in Western Blot (WB), Immunocytochemistry (ICC/IF) and Immunohistochemistry (IHC) applications. This … WebHuman Phospho-FAK (Y397) Antibody Summary Species Reactivity Human Specificity Detects human FAK when phosphorylated at Y397. Source Polyclonal Rabbit IgG Purification Antigen and protein A Affinity-purified Immunogen Phosphopeptide containing human FAK Y397 site Formulation Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
WebPVDF membrane was probed with 0.5 µg/mL of Mouse Anti-Human Phospho-FAK (Y925) Monoclonal Antibody (Catalog # MAB44671) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007 ). A specific band was detected for Phospho-FAK (Y925) at approximately 125 kDa (as indicated). This experiment was conducted under …
Web# V9131) and anti-tyrosin 397-phospho-focal adhesion kinase (FAK) (rabbit polyclonal, Fisher Scientific, Cat. # 700255) specific primary antibodies diluted 1:300 and 1:500, respectively, in blocking solution at room temperature for 1 hour by placing samples upside-down on a drop (50 µL) of antibody solution. After washing in 1xPBS cells were ... fnf life on hiatusWebPhospho-FAK (Tyr576) Antibody detects endogenous levels of FAK only when phosphorylated at Tyrosine 576. RRID: AB_2834828 Cite Format: Affinity Biosciences Cat# AF3397, RRID:AB_2834828. Conjugate: Unconjugated. Purification: The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho … green valley az 55+ active communitiesWeb锌指蛋白925抗体的相关产品：phospho-Tau (Thr548)中文名称: 磷化微管相关蛋白抗体AFP中文名称: ... green valley az ambulance serviceWebABT135 Sigma-Aldrich Anti-phospho-FAK (Tyr397) Antibody Download Zoom Detect phospho-FAK (Tyr397) using this Anti-phospho-FAK (Tyr397) Antibody validated for use … green valley az 55+gated communitiesWebFAK (phospho Tyr397) antibody detects FAK (phospho Tyr397) protein at cell junction, nucleus and cytoplasm by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: FAK (phospho Tyr397) stained by FAK (phospho Tyr397) antibody (GTX129840) diluted at 1:500. green valley az average weatherWebMar 23, 2024 · Preparation of rabbit anti-phospho-FAK (Tyr397) antibody: Briefly spin the vial 6. of rabbit anti-phospho-FAK (Tyr397) (Item C-1). Add 100 µl of 1X Assay Diluent into the vial to prepare a phospho detection antibody concentrate. Pipette up and down to mix gently (the concentrate can be stored at 4°C for 5 green valley az air conditioningWebPhospho-FAK (Tyr397) Antibody detects endogenous levels of FAK only when phosphorylated at Tyrosine 397. RRID: AB_2834829 Cite Format: Affinity Biosciences Cat# AF3398, RRID:AB_2834829. Conjugate: Unconjugated. Purification: The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho … green valley az 55+ communities