WebQu tackled this problem by using cell lysis aids to rapidly load the fluorescent probe into pollen tubes at room temperature, reducing the loading time to 15 mins. [167] After 5 mins of loading with cell lysate containing Fluo-4-AM, the Ca 2+ gradient from the tip to the bottom of the pollen tube was not particularly pronounced. WebNov 30, 2024 · Removal of PCB-containing FLBs, as part of lighting upgrades or a stand-alone project, is an investment that may pay off with long-term benefits to students, school staff, the community, and the environment. TSCA Storage Disposal Requirements for Fluorescent Light Ballasts (pdf) (56.21 KB)
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