WebThe HaloTag is typically used for cell-based/imaging assays such as immunofluorescence immunoprecipitation protein purification. Which ligands are available? There are … WebHaloTag® Technology: A Powerful Tool for Protein Labeling and Analysis Start by adding the HaloTag® protein fusion tag to your protein, pair the fusion protein with one of many interchangeable ligands that forms a highly specific covalent bond with HaloTag, and you …

HaloTag, a Platform Technology for Protein Analysis

WebThis tag is used for protein purification of recombinant proteins and its fragments. It can be used in research labs and it is intended for large-scale purification during downstream manufacturing process as well. The iCapTag™-target protein complex can be expressed in a wide range of expression hosts (e.g. CHO and E.coli cells). Web2 Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA · Toll Free in USA 800-356-9526 · 608-274-4330 · Fax 608-277-2516 TM348 · Revised 6/16 … safety food certificate

Affinity Tags for Protein Purification - PubMed

Webcombination with a second tag it can be an interesting tool for protein purification. Polyhistidine-tag (His-tag) A widely employed method utilizes immobilized metal-affinity chromatography to purify recombinant proteins containing a short affinity-tag consisting of polyhistidine residues. Immobilized metal-affinity chromatography WebUse the HaloTag® Protein Purification System for: Purification of poorly expressed fusion proteins; Improved yield, purity and specific activity of soluble, functional proteins compared to His-Tag, GST and MBP … WebApr 3, 2024 · The purity and yields were significantly higher for protein purified using HaloTag, ≥95% purity and ≥86% yield, in comparison to the other tags (Fig 1B). After successful protein purification, the HaloTag-p65 protein fusion was used to try to isolate known protein interactors: NFκB1 (p50, p105), NFκB2 (p52, p100), RelA (p65), RelB … the wren magazine